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4 votes
1 answer
85 views

split fastq file containing a sequence block at different locations

6 votes
3 answers
862 views

Relationship between sequencing lane and ngs dataset

10 votes
2 answers
2k views

How do you generate read-length vs read-quality plot for long-read sequencing data (e.g., MinION)?

2 votes
1 answer
33 views

Approaches for filtering bacterial/fungal contaminant sequences from RADseq results

1 vote
1 answer
87 views

Lower case vs. upper case nucleotids in sequence vs. dots at the end

2 votes
1 answer
70 views

Sanger sequencing annotation error

2 votes
1 answer
219 views

calculate mismatch frequency/rate from a BAM file

0 votes
0 answers
24 views

Suggestion for way foward with upregulated genes

1 vote
1 answer
67 views

What are the output files of RNA-Seq from facility?

1 vote
1 answer
73 views

issue with rna seq analysis

2 votes
1 answer
112 views

Is it common to get different number of SNVs+Indels across samples from vcf files generated using GATK and DRAGEN (counts are higher for GATK)?

0 votes
1 answer
29 views

Generating simulated gold standard NGS dataset/ Any publicly available smal gold standard NGS dataset

2 votes
1 answer
55 views

How to interpret a GenomeScope plot?

1 vote
1 answer
137 views

Kraken2 Standard Database failing to build (unexpected FTP path)

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