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I am trying to build a features matrix to be used for Random Forest based classification. I'd like to add, as features, short motifs which are common to all the protein sequences belonging to a specific gene family.

I tried to use MEME but I'd like to know if there are also other good tools (online or command line). Keep in mind I have more than 10.000 sequences and that I prefer short motifs (3/4 aa long).

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I think MEME is a good tool for your purpose, but there are others as well. I can think of InterProScan for example (although I am not sure if it is really de novo). Here a summary of available tools.

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