Top new questions this week:
|
|
I have a FASTA file which I would like to convert into FASTQ format as the tool I want to use my data in requires it in FASTQ format. So dummy quality scores are fine.
Note: I am not using any ...
|
|
I wrote a script which should changes IDs in a GFF3 file. Unfortunately, the below script has two problems.
It attaches the new ID to Parent which leads that Parent contains the old and new id. How ...
|
Greatest hits from previous weeks:
|
|
I want to get a .bed file with the genes' names and canonical coordinates, also I would like to have coordinates of exons, too. I can get the list from UCSC, however, if I choose UCSC Genes - ...
|
|
I recently installed Ubuntu 16.04 (because I was still using 12.04). But it seems my bedtools scripts don't work properly anymore. I can't figure out how to use the new bedtools for my old ways. What ...
|
|
I'd like to learn the differences between 3 common formats such as FASTA, FASTQ and SAM. How they are different? Are there any benefits of using one over another?
Based on Wikipedia pages, I can't ...
|
|
I am currently exploring the BLAST program, just for testing purpose i generated two FASTA files, containing two genes A and B, such that B is just a motif of repeated 'G's that occurs in A.
file ...
|
|
I have 10x single cell RNA seq data. Which R package is best suited for analysis of the 10x data matrix. What is the script to prepare the data for downstream GSEA analysis.
I have already processed ...
|
|
I have a fasta file like this:
>Id1
ATCCTT
>Id2
ATTTTCCC
>Id3
TTTCCCCAAAA
>Id4
CCCTTTAAA
I want to delete sequences that have the following IDs.
Id2
Id3
The IDs are in a .txt file, ...
|
|
I have read counts data and I want to convert them into RPKM values. For this conversion I need the gene length.
Does the gene length need to be calculated based on the sum of coding exonic lengths? ...
|
