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Lin
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I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as follows:

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol https://satijalab.org/seurat/v3.1/immune_alignment.html said, I need to use SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")

I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as follows:

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol said, I need to use SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")

I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as follows:

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol https://satijalab.org/seurat/v3.1/immune_alignment.html said, I need to use SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")
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terdon
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Anyone can help me? I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as following,follows:

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol said, I need to use SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")

Anyone can help me? I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as following,

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol said, I need to use SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")

I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as follows:

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol said, I need to use SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")

Anyone can help me? I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as following,

Control.data <- Read10X("~/Documents/... Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol said, I need to use "SplitObject"SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")

Object_list <- SplitObject(Object, split.by = "?")

Anyone can help me? I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as following,

Control.data <- Read10X("~/Documents/... Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol said, I need to use "SplitObject" function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")

Anyone can help me? I want to do comparative analyses of my two individual single cell RNA-Seq datasets in Seurat. And I load and list my data as following,

Control.data <- Read10X("~/Documents/...  Control_filtered_feature_bc_matrix")

Control <- CreateSeuratObject(counts  = Control.data, project = "Control_summary", min.cells = 3, min.features = 200)

Mut.data <- Read10X("~/Documents/... /Mut_filtered_feature_bc_matrix")

Mut <- CreateSeuratObject(counts  = Mut.data, project = "Mut_summary", min.cells = 3, min.features = 200)

Object<- list(Control,Mut)

names(Object) <- colnames(Object)

Then the protocol said, I need to use SplitObject function, but what do I split by?

Object_list <- SplitObject(Object, split.by = "?")
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Lin
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