Very simple set-up: We have PacBio long high-fidelity (HiFi) reads, genome sequencing, and some of those that contain a particular sequence. I want to find out which ones. The length of the query sequence is 7kb, though ideally I would use as query a sub-sequence of only 1.5kb.

The output can the just the read ID or the full read sequence.

We tried DAMAPPER and it gives some decent results but it might not be appropriate to search for the shorter sub-sequence of 1.5kb. It also feels like an overkill for this purpose.

Bare in mind that this is my first foray in long-read sequencing so my mindset is still very much one of short low error-rate reads.

Very simple set-up: We have a PacBio long high-fidelity (HiFi) reads, genome sequencing, and some of those that contain a particular sequence. I want to find out which ones. The length of the query sequence is 7kb, though ideally I would use as query a sub-sequence of only 1.5kb.

The output can the just the read ID or the full read sequence.

We tried DAMAPPER and it gives some decent results but it might not be appropriate to search for the shorter sub-sequence of 1.5kb. It also feels like an overkill for this purpose.

Bear in mind that this is my first foray into long-read sequencing so my mindset is still very much one of short, low error-rate reads.