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Jul 6, 2020 at 19:14 comment added M__ .. me neither but they are doing Plasmodium (malaria, P. falciparum?) so there will be 24-hour periodicity in the RNA expression depending on what in vitro assay they are doing. That would make it a bit complicated. They also appear to do martial arts - its a tough field malaria ;-)
Jul 6, 2020 at 17:49 comment added StupidWolf good suggestions @Michael. It's not a big dataset, 3500+ cells, so you can do PCA or tSNE on the dimension reduced data. I don't see the OP's calculation though, or what is the larger question..
Jul 6, 2020 at 16:10 comment added M__ I prefer @haci 's work around, thanks are not a cool way to say thanks.but anyway
Jul 6, 2020 at 15:10 comment added Spartan 117 Even PCA gives problem if it ingests this sce-object. Anyway I solved changing the computer with one more performative. Very good to know that GCP is free for three months. Thank you!
Jul 6, 2020 at 11:35 history edited M__ CC BY-SA 4.0
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Jul 6, 2020 at 11:11 history edited M__ CC BY-SA 4.0
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Jul 6, 2020 at 10:58 history answered M__ CC BY-SA 4.0