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gringer
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Answer from @ATpoint, converted from comment:

A detected gene is one that has a count > 0, and generally the deeper you sequence the more genes you detect until you reach a saturation which is being influenced by the number of biologically-expressed genes and the single-cell technology you're using, e.g. droplet vs plate-based and the library setup, e.g. end-tagged vs full length.

Counts cannot influence the nature of gene translation. A count is an observation; a translated gene sequence is a physical thing. Whether or not a gene can be translated into protein has no relationship to how often it is observed in existence.

Even with count zero (i.e. no detection in scRNA-seq), it does not mean that the gene is not present in the cell. NGS technology is a relative quantification, I doubt it is meaningful to try to directly make statements about the connection between read counts and biological meaning in the cell.

Answer from @ATpoint, converted from comment:

A detected gene is one that has a count > 0, and generally the deeper you sequence the more genes you detect until you reach a saturation which is being influenced by the number of biologically-expressed genes and the single-cell technology you're using, e.g. droplet vs plate-based and the library setup, e.g. end-tagged vs full length.

Counts cannot influence the nature of gene translation. A count is an observation; a translated gene sequence is a physical thing. Whether or not a gene can be translated into protein has no relationship to how often it is observed in existence.

Answer from @ATpoint, converted from comment:

A detected gene is one that has a count > 0, and generally the deeper you sequence the more genes you detect until you reach a saturation which is being influenced by the number of biologically-expressed genes and the single-cell technology you're using, e.g. droplet vs plate-based and the library setup, e.g. end-tagged vs full length.

Counts cannot influence the nature of gene translation. A count is an observation; a translated gene sequence is a physical thing. Whether or not a gene can be translated into protein has no relationship to how often it is observed in existence.

Even with count zero (i.e. no detection in scRNA-seq), it does not mean that the gene is not present in the cell. NGS technology is a relative quantification, I doubt it is meaningful to try to directly make statements about the connection between read counts and biological meaning in the cell.

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gringer
  • 15.1k
  • 5
  • 24
  • 83

Answer from @ATpoint, converted from comment:

A detected gene is one that has a count > 0, and generally the deeper you sequence the more genes you detect until you reach a saturation which is being influenced by the number of biologically-expressed genes and the single-cell technology you're using, e.g. droplet vs plate-based and the library setup, e.g. end-tagged vs full length.

Counts cannot influence the nature of gene translation. A count is an observation; a translated gene sequence is a physical thing. Whether or not a gene can be translated into protein has no relationship to how often it is observed in existence.

Answer from @ATpoint, converted from comment:

A detected gene is one that has a count > 0, and generally the deeper you sequence the more genes you detect until you reach a saturation which is being influenced by the number of biologically-expressed genes and the single-cell technology you're using, e.g. droplet vs plate-based and the library setup, e.g. end-tagged vs full length.

Answer from @ATpoint, converted from comment:

A detected gene is one that has a count > 0, and generally the deeper you sequence the more genes you detect until you reach a saturation which is being influenced by the number of biologically-expressed genes and the single-cell technology you're using, e.g. droplet vs plate-based and the library setup, e.g. end-tagged vs full length.

Counts cannot influence the nature of gene translation. A count is an observation; a translated gene sequence is a physical thing. Whether or not a gene can be translated into protein has no relationship to how often it is observed in existence.

Source Link
gringer
  • 15.1k
  • 5
  • 24
  • 83

Answer from @ATpoint, converted from comment:

A detected gene is one that has a count > 0, and generally the deeper you sequence the more genes you detect until you reach a saturation which is being influenced by the number of biologically-expressed genes and the single-cell technology you're using, e.g. droplet vs plate-based and the library setup, e.g. end-tagged vs full length.

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