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Aug 8, 2023 at 21:45 comment added M__ Thanks @SylviaRodriguez I'm glad its resolved.
Aug 8, 2023 at 21:38 vote accept Sylvia Rodriguez
Aug 8, 2023 at 21:38 comment added Sylvia Rodriguez Thank you. I like that solution. Two separate analyses.
Aug 7, 2023 at 3:02 comment added M__ @SylviaRodriguez addressed in the comments
Aug 7, 2023 at 3:02 history edited M__ CC BY-SA 4.0
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Aug 6, 2023 at 22:25 comment added Sylvia Rodriguez Thank you for your feedback M__. Yes, this operation selects all genes with >2 and all genes with <-2 fold change in expression. However, none of the values are themselves retained. It is only the names of the genes that are taken forward to the next step. Therefore, the direction (- or +) is lost after this step. In other words, if you have gene A +8 fold change, gene B +3 and gene C +2.4, then the end result is the same if gene A had been -6.2 fold change, gene B -2.4 and gene C +2.1. I suppose I conclude that we are looking at representation, rather than at up/downregulated pathways?
Aug 6, 2023 at 22:10 history answered M__ CC BY-SA 4.0