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Oct 9, 2023 at 0:36 comment added Ammar Agree with what @rainman has said. depending on the organism, you might want to go lower, eg if the genome is is tiny you might drop the cutoff. Also it's good to note there are scaffolding tools that help bridge the gap between contigs.
Oct 6, 2023 at 19:20 comment added Rainman Oh and for the cutoffs: I work with metagenomes, so I tend to work my cutoff around my assembly N50. Say the N50 is 2100, I will round that to 2000 and remove any scaffolds smaller than that. It seems to help me save the majority of the data whilst minimizing noise. Each case will be different though :)
S Oct 6, 2023 at 18:11 review First answers
Oct 11, 2023 at 8:09
S Oct 6, 2023 at 18:11 history edited Rainman CC BY-SA 4.0
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S Oct 6, 2023 at 17:08 review First answers
Oct 6, 2023 at 17:57
S Oct 6, 2023 at 17:08 history answered Rainman CC BY-SA 4.0