I am loading cellranger data into seuratobject as following, but it keeps showing Error in "Barcode file missing. Expecting barcodes.tsv.gz"

the running code and result:

> #clean up your environment
> rm(list=ls())

> # Load required libraries
> library(Seurat)
> library(dplyr)

> # Load required library for reading Matrix Market files
> library(Matrix)

> # For output from CellRanger >= 3.0 with multiple data types
> data_dir <- 'C:/Users/Administrator/Desktop/10x_raw'

> list.files(data_dir) # Should show barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz
[1] "GSM7494257_AML16_DX_raw_barcodes.tsv.gz" "GSM7494257_AML16_DX_raw_genes.tsv.gz"   
[3] "GSM7494257_AML16_DX_raw_matrix.mtx.gz"  

> data <- Read10X(data.dir = data_dir)
Error in Read10X(data.dir = data_dir) : 
  Barcode file missing. Expecting barcodes.tsv.gz
 
> seurat_object = CreateSeuratObject(counts = data$`Gene Expression`)
Error in data$`Gene Expression` : 
  object of type 'closure' is not subsettable

I am sure this file inside the directory. Dose anyone know what's the issue? Thanks in advance.

I am loading cellranger data into seuratobject as following, but it keeps showing Error in

Barcode file missing. Expecting barcodes.tsv.gz

the running code and result:

#clean up your environment
rm(list=ls())

# Load required libraries
library(Seurat)
library(dplyr)

# Load required library for reading Matrix Market files
library(Matrix)

# For output from CellRanger >= 3.0 with multiple data types
data_dir <- 'C:/Users/Administrator/Desktop/10x_raw'

list.files(data_dir) # Should show barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz
[1] "GSM7494257_AML16_DX_raw_barcodes.tsv.gz" "GSM7494257_AML16_DX_raw_genes.tsv.gz"   
[3] "GSM7494257_AML16_DX_raw_matrix.mtx.gz"  

data <- Read10X(data.dir = data_dir)
Error in Read10X(data.dir = data_dir) : 
  Barcode file missing. Expecting barcodes.tsv.gz

seurat_object = CreateSeuratObject(counts = data$`Gene Expression`)
Error in data$`Gene Expression` : 
  object of type 'closure' is not subsettable

I am sure this file inside the directory. Dose anyone know what's the issue?

Thanks in advance.

I am loading cellranger data into seuratobject as following, but it keeps showing Error in "Barcode file missing. Expecting barcodes.tsv.gz"

the running code:

#clean up your environment rm(list=ls())

Load required libraries

library(Seurat) library(dplyr)

Load required library for reading Matrix Market files

library(Matrix)

For output from CellRanger >= 3.0 with multiple data types

data_dir <- 'C:/Users/Administrator/Desktop/10x_raw' list.files(data_dir) # Should show barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz [1] "GSM7494257_AML16_DX_raw_barcodes.tsv.gz" "GSM7494257_AML16_DX_raw_genes.tsv.gz"
[3] "GSM7494257_AML16_DX_raw_matrix.mtx.gz"
data <- Read10X(data.dir = data_dir) Error in Read10X(data.dir = data_dir) : Barcode file missing. Expecting barcodes.tsv.gz seurat_object = CreateSeuratObject(counts = data$`Gene Expression`) Error in data$Gene Expression : object of type 'closure' is not subsettable

I am sure this file inside the directory. Dose anyone know what's the issue? Thanks in advance.

I am loading cellranger data into seuratobject as following, but it keeps showing Error in "Barcode file missing. Expecting barcodes.tsv.gz"

the running code and result:

> #clean up your environment
> rm(list=ls())

> # Load required libraries
> library(Seurat)
> library(dplyr)

> # Load required library for reading Matrix Market files
> library(Matrix)

> # For output from CellRanger >= 3.0 with multiple data types
> data_dir <- 'C:/Users/Administrator/Desktop/10x_raw'

> list.files(data_dir) # Should show barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz
[1] "GSM7494257_AML16_DX_raw_barcodes.tsv.gz" "GSM7494257_AML16_DX_raw_genes.tsv.gz"   
[3] "GSM7494257_AML16_DX_raw_matrix.mtx.gz"  

> data <- Read10X(data.dir = data_dir)
Error in Read10X(data.dir = data_dir) : 
  Barcode file missing. Expecting barcodes.tsv.gz

> seurat_object = CreateSeuratObject(counts = data$`Gene Expression`)
Error in data$`Gene Expression` : 
  object of type 'closure' is not subsettable

I am sure this file inside the directory. Dose anyone know what's the issue? Thanks in advance.