I recently used pbmm2 to align my reads to the reference genome, when I specified --sort option it is generated a file with extension bam.bai. Afterwards, an index with extension .bam.pbi was generated with pbindex. IGV could not open .bam.pbi, so I tried with bam.bai. The first time I could see the mapping, but I have tried the same steps as the first time and I cannot see the coverage of the reads.

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I recently used pbmm2 to align my reads to the reference genome, when I specified --sort option it is generated a file with extension bam.bai. Afterwards, an index with extension .bam.pbi was generated with pbindex. IGV could not open .bam.pbi, so I tried with bam.bai. The first time I could see the mapping, but I have tried the same steps as the first time and I cannot see the coverage of the reads.

I recently used pbmm2 to align my reads to the reference genome, when I specified --sort option it is generated a file with extension bam.bai. Afterwards, an index with extension .bam.pbi was generated with pbindex. IGV could not open .bam.pbi, so I tried with bam.bai. The first time I could see the mapping, but I have tried the same steps as the first time and I cannot see the coverage of the lectures.

I recently used pbmm2 to align my reads to the reference genome, when I specified --sort option it is generated a file with extension bam.bai. Afterwards, an index with extension .bam.pbi was generated with pbindex. IGV could not open .bam.pbi, so I tried with bam.bai. The first time I could see the mapping, but I have tried the same steps as the first time and I cannot see the coverage of the reads.