Firstly you wanted a webserver to build a tree, ok, I would use PhyML server. The good thing is that with only 22 taxa the calculations are easily doable.
What I'm assuming is you can map your rearrangements to produce a single homologous gene alignment for all taxa. So you appear to infer you can break your 20-30kb contig into individual aligments of homologous genes which has incorporated the rearrangement and enables easy production of an alignment file. This would leave you with around 20-30 gene alignment files. I hope that is correct, because that is how you get around the rearrangement problem and enables the data to be reassembled for the final tree (below).
Doing the tree is simple, because you can exploit the assumptions used in tree building regarding independence between mutations at each alignment position. The theoretical proof, the data is sound, is complicated. In summary,
- build a consensus phylogeny (one tree representative of lots of trees) using two different approaches
- combine the data into a single alignment, if the above looks good, or excluding the alignments that are affected recombination.
The theoretical issues with what you want to do are complicated because you need to distinguish bifurcating (clonal) homologues from recombination that could have occured in the reassortment events. The following is a purists approach, you might not want to do this stuff, but it gives you some idea.
Just before I do that ... I just want to check the alignments assumption is correct? and which bacteria?
