So this is related to CRISPR-CAS9. I am working with off-target predictions for my thesis and was looking at all scientific papers related to CRISPR. I found one and decided to use their datasets. The datasets were not available directly and the method used to obtain them was specified which I had mentioned below:
They have around 30 sgRNAs which are each 23bp long and are aligning the sgRNAs to human reference genome with a tolerance of upto 6 mismatches using Bowtie2.
In order to access the exact dataset, I thought I need to replicate the method they used. To do so, I collected the sgRNAs from their paper and tried to use Bowtie2 to align them against human genome with upto 6 mismatches.
Since my laptop does not have the capacity to perform this operation (it has 8GB RAM), I am searching for interfaces that provide access to Bowtie2. I found Galaxy to be useful. Does anyone know of any other tool that provides an option for Bowtie2?