When reading a paper, I came across the following statement
Standard vectors were constructed, flanked by pairs of homology sequences derived from yeast barcodes at the ends of each expression cassette. We reasoned that as these barcode sequences were designed to be orthogonal, they could serve a dual purpose of reducing the probability of mis-annealing and dictating the assembly order of multiple cassettes.
I am not sure what the "orthogonal" means here. I think it is about synthetic biology.