“The Iceman” was a man who lived 5300 years ago and whose body was recovered from an Alpine glacier in 1991. Some fungal material was recovered from his clothing and sequenced.
Ice man : found as a frozen corpse in the Val Senales Glacier in Italy.
Thought to be carrying Fomitopsis betulina (formerly known as Piptoporus betulinus) commonly known as birch polypore, birch bracket, or razor strop) is a common Basidiomycota brown rot macrofungus growing on decaying birch wood.
Other species known : Fomes fomentarius (commonly known as the tinder fungus, false tinder fungus, hoof fungus,] tinder conk, tinder polypore or ice man fungus)
Given two DNA fragments from NCBI genbank, try to find what species these DNA fragments belongs to or what species these fragments are most closely related to.
>Z54169.1 Iceman fungal clone 'Sim C-a1' 18S rRNA gene (partial) GTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAAACTTGGGCCTGGCTGGCAGGTCCGCCTCAC CGCGTGTACTTGTCCGGCCGGGCCTTTCCTTCTGGAGAACCTCATGCCCTTCACTGGGTGTGTTGGGGAA CCAGGACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTTTGCTCGAATACGTTAGCATGGAAT AATAGAATAGGACGTGCGGTCCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGACAGTCG GGGGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGAAGACTAACTACTGCGAAAGCAT TTGCCAAGGATGTTTTCATTAATCAGTGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAG TCTTAACCGTAAACTATGCCGACTAGGGATCGGGCGATGTTCTTTTTCTGACTCGCTCGGCACCTTACGA GAAATCAAAGTTTTTGG
>Z54155.1 Iceman fungal clone 'Sim C-a2' 18S rRNA gene (partial) GTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGAACCTTGGGTCTGGCTGGCAGGTCCGCCTCAC CGCGTGTACTTGTCCGGCCGGGCCTTCCTTCTGGAGAACCTCATGTCTTTTATTAGGCGTGTTGGGGACC AGAACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCTTTGCTCGAATACGTTAGCATGGAATAA TAGAATAGGACGTGCGGTCCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGACAGTCGGG GGCATCAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTT GCCAAGGATGTTTTCATTAATCAGTGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTC TTAACCGTAAACTATGCCGACTAGGGATCGGGCGATGTTCTTTTTCTGACTCGCTCGGCACCTTACGAGA AATCAAAGTTTTTGG
Now I know I can just google what species of mushrooms (which is where the above background info is from). But say that I was unable to and had to just go off the DNA fragments in order to find the species.
BLASTn, but I'm not sure if it is the right tool or if I need to properly calibrate the parameters to use it.
so far, I've tried:
Under “Choose Search Set: Database : Non-redundant protein sequences (nr) Organisms excluded: Animalia (taxid:33208), Plantae, Prokaryota (taxid:2), Protozoa Organisms included: fungi (taxid:4751) Included: Models (XM/XP), Uncultured/environmental sample sequences Under Program Selection: Optimized for Highly similar sequences (megablast) Algorithm Parameters (General Parameters, Scoring Parameters, Filters and Masking, PS/PHI?DELTA BLAST) are all kept default. ```