# how can i calculate p value of miRNA raw reads counts using R?

working on lung cancer project and need to find out which genes are deferentially expressed and which are not. for that i a have downloaded miRNA data set from GEO id is GSE110907. And the file i got has to variable gene_id and read counts. from that i have to derive p value from that data/file.

• For context, this question was already asked at biostars biostars.org/p/417111, with some follow-up comments. Jan 20 '20 at 10:52

DEA has been addressed by many researchers and R has a lot of packages to help you with this; DESeq2, edgeR, limma, etc ...
I would go to the Biconductor website, search for one of the three above, and go through their rich vignettes and would come back here for specific questions.