Sorry if this has been asked before, I've done a quick search and I don't think there's an easy explanation for me to understand.
I'm really new to bioinformatics/RNASEQ analysis, having only taken up an attempt to process my RNASEQ data last week. I have 0 background and some rudimentary knowledge of command line. So far I've managed to get access to my universities HPC and perform a fastqc on my fastq data.
What I'm stuck at at the moment is attempting to trim my RNASEQ data and remove adapters. My data had been run through Novaseq and I'm not exactly sure how to trim my current RNASEQ data.
I've attempted to run the following script using trimmomatic
# go to working folder cd /project/CHR_RNASEQ/reads # run trimmomatic trimmomatic SE -threads 8 -phred33 SLIDINGWINDOW:4:20 ILLUMINACLIP:$TRIM/adapters/TruSeq3-SE-2.fa:2:30:10 MINLEN:36 1_R1.fastq.gz 1_R1_trimmed.fastq.gz
It did log a job in the HPC, but I had no output at the end. I didn't receive an error either. This is unfortunately just some code I had lifted and trialed and errored until something finally ran, but I'm very sure I'm very wrong and any help with understanding this would be really appreciated.