I need to merge my all transcripts.gtf from cufflinks output follow this command line :
cuffmerge -o merged_gtf_output -p 15 -s ref.fasta -g anot.gtf assembly.txt
But I have this warning :
[bam_header_read] EOF marker is absent. The input is probably truncated. [bam_header_read] invalid BAM binary header (this is not a BAM file). File merge_B3//tmp/mergeSam_fileOaNffV doesn't appear to be a valid BAM file, trying SAM... [21:40:04] Loading reference annotation. [21:40:05] Inspecting reads and determining fragment length distribution. Processed 3921 loci. > Map Properties: > Normalized Map Mass: 33242.00 > Raw Map Mass: 33242.00 > Fragment Length Distribution: Truncated Gaussian (default) > Default Mean: 200 > Default Std Dev: 80
Can someone tell me what this warning means and how to fix it?