Hope everyone is well.
So i was appointed with a task, and i need some guidance. Attention, the one that gave me the task may not have explained this very clearly. I will write what he exactly asked of me, and what he gave me as primary data.
So he gave me 4 bed files (human genome) with chr and coordinates(start/stop), and asked me to do: "sequencing/coordinates conservation between diverse species".
Is that doable with coordinates only or do i need sequences in fasta format (phast uses fasta format)? I just dont understand how i will find "conservation between diverse species" only with coordinates. It seems weird to me.
I have found these programs: TargetOrtho2, Phast and BUSCO(?). Are they any good? Should i continue with one of them that you may have experience with?