i have created a phmm in hmmer from a multiple alignment focused on E. coli produced in tcoffee from sequences taken from blast similar to my query sequence.
- I would like to ask how i can compare the results from HMMER for a pHMM against UNIPROT (i used the phmm that i created and i searched in hmmer search against uniprot) and the results from BLAST.
- I would like to ask if i can search a pHMM in BLAST somehow, or if blast accepts only fasta one sequence.
- Why e-values of hmmer and blast are not comparable??Why e-value in hmmer is never 0? Thank you so much!!