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i need to search for one protein against UNIPROT in BLAST for a selected organism. How can I do it? (SWSISSPROT+TREMBL) THANKS!!

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I don't know of any online tool enabling a search against TrEMBL. You probably will have to set up your own local BLAST environment, roughly following these steps:

  1. download blast+ - but there might be an easier way to install the software, depending on your operating system (e.g. on Ubuntu you can just sudo apt install ncbi-blast+)
  2. download target databases
  3. build local database

I hope this helps.

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  • $\begingroup$ thank you a lot for your answer!unfortunately my programming skills are not so strong! $\endgroup$
    – marilu
    Commented Apr 25, 2020 at 20:58
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UniProt provides a BLAST service (https://www.uniprot.org/blast) which you can run against several UniProt databases, or you could use the Expasy BLAST too, at https://web.expasy.org/blast.

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