# how can i search in p-blast against UNIPROT(not only swissprot)

i need to search for one protein against UNIPROT in BLAST for a selected organism. How can I do it? (SWSISSPROT+TREMBL) THANKS!!

I don't know of any online tool enabling a search against TrEMBL. You probably will have to set up your own local BLAST environment, roughly following these steps:

1. download blast+ - but there might be an easier way to install the software, depending on your operating system (e.g. on Ubuntu you can just sudo apt install ncbi-blast+)
3. build local database

I hope this helps.

• thank you a lot for your answer!unfortunately my programming skills are not so strong! Apr 25 '20 at 20:58

UniProt provides a BLAST service (https://www.uniprot.org/blast) which you can run against several UniProt databases, or you could use the Expasy BLAST too, at https://web.expasy.org/blast.