I'm using Primer-Blast to compare a PCR template against a custom database of reference sequences, using Primer-Blast's Custom database functionality.
However whenever I try to upload a FASTA file as the custom database and then run Primer-Blast, I get the following error:
Exception error: Fasta Reader: sequence id ends with 80 valid nucleotide characters. Was the sequence accidentally placed in the definition line?
No matter how I try to format my FASTA file, I get this error.
Does anyone know how to properly format a FASTA file to have it accepted as a custom database of reference sequences to compare against the PCR template? Or is this a bug in the software?
Here's an example FASTA file, to upload both for the template and the custom database.
>test1 ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAA AATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGG ACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTT CGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGC