I want to use a local
blastn command to BLAST a multifasta file ~700 sequences using following command:
blastn -db nt -query fasta_all.fasta -num_alignments 2 -out fasta_blasted.txt but I receive error :
BLAST Database error: Error: Not a valid version 4 database.
I use a local
nt database which I downloaded today (11th of May 2020) by running
update_blastdb.pl --decompress nt [*]. I downloaded this but I did not do any post-processing and just the "raw" download files are in the folder (see image below which shows only a part of the files)
blast+ I downloaded and installed is
ncbi-blast-2.10.0+-x64-linux.tar.gz from https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/
So, both database and
blastn version should be the latest. It probably has something to do with either the version of the database or the software, but as it both are the latest version, I find it strange that these would not be compatible. Any ideas what causes this error?