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I need to calculate Isoelectric point from a multifasta file, is there any python code or web tool that allows me to do that?

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  • $\begingroup$ Can you use Python (biopython has a very straightforward method for pI calculations, Bio.SeqUtils.IsoelectricPoint)? $\endgroup$ Jul 13 '20 at 9:59
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You can use EMBOSS Pepstats for this. It takes a multi-fasta as input and produces a table that contains various statistics related to the protein, including isoelectric point.

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