# Question of Padj value (very high, infinite) got from dds

After I got dds from my count matrix by DESeq(), I use results() method to get Padj and log2 fold changes for volcano plotting, then I found some gene's Padj values are infinite and the P-value is very close to 0; this makes those dots on my volcano plotting located very top to Y-aix and also confused me:

1. is this normal or not, such a p-value (very close to 0) and -logPadj is infinite;
2. how should I treat those data: are they very different expressed between samples and control or just abnormal data which should be taken off?

Here follows the table:

table 1 is dds results, -log10(padj) show inf; table 2 is count matrix from featureCount table 3 is size factor of samples

• Agreed. If you want to get rid of Inf you can add a tiny number to the pvalues before the conversion, e.g. p + .Machine\$double.xmin. – ATpoint Nov 27 '20 at 17:34