After I got dds from my count matrix by DESeq(), I use results() method to get Padj and log2 fold changes for volcano plotting, then I found some gene's Padj values are infinite and the P-value is very close to 0; this makes those dots on my volcano plotting located very top to Y-aix and also confused me:
- is this normal or not, such a p-value (very close to 0) and -logPadj is infinite;
- how should I treat those data: are they very different expressed between samples and control or just abnormal data which should be taken off?
table 1 is dds results, -log10(padj) show inf; table 2 is count matrix from featureCount table 3 is size factor of samples
Please help me !