I have these Ct data from qPCR for a series of samples A-H, I want to quantify the RNA copies of my samples using R:
Sample_ID Replicate1_Ct Replicate2_Ct
A 22.92 24.21
B 24.29 22.16
C 24.29 22.16
D 27.91 23.76
E 21.44 22.81
F 16.50 18.31
G 17.79 16.56
H 17.34 19.09
In addition, I have measured a standard (STD) curve of copy number of the RNA in question, that I want to use as a reference:
STD_Ct STD_copies Log10(STD_copies)
33 4.00E+03 3
28 4.00E+04 4
24 4.00E+05 5
20 4.00E+06 6
15 4.00E+07 7
12 4.00E+08 8
It seems