This figure 2.Here they are doing.
They here they are comparing different cell type and the organ type and which they showing the ontology enrichment.
The method they have used for RNA seq
Cell-type-specific and organ-specific marker genes were identified using a two-step procedure. First, we performed pairwise differential
expression analysis for each of the three type of structural cells, comparing them across organs. Second, based on the resulting pairwise comparisons, we identified for each organ those genes that were upregulated in each cell type compared to at least five other organs. Pairwise comparisons between organs were performed using the limma package in R, separately for each of the three structural cell types. Significantly differential genes were selected based on statistical significance (adjusted P < 0.05), average expression (log2(CPM) > −1) and sequencing coverage (median number of reads greater than 10 in the group with stronger signal). On the basis of these pairwise comparisons, we counted the total number of times each gene was upregulated in a specific organ compared to all other organs. Genes that were upregulated in comparison to five or more other organs were selected as marker genes of the corresponding organ.
Now how this part is being done ? "Genes that were upregulated in comparison to five or more other organs were selected as marker genes of the corresponding organ.*"
On the basis of these pairwise comparisons, we counted the total number of times each gene was upregulated in a specific organ compared to all other organs. Genes that were upregulated in comparison to five or more other organs were selected as marker genes of the corresponding organ.