# Why could I be having only NAs for p_val and p_val_adj in Seurat DGE analysis?

I am doing a differential gene expression analysis to find out genes that are differentially expressed in one cell type from the lung in two conditions, control and idipathic pulmonary fibrosis (IPF). In total I have 152538 cells. Can anyone help me provide a hint to understand why only NAs get returned for the pvalues and adjusted pvalues?

> table(seurat\$group)

Control     IPF
64652   87886

> degs <- FindMarkers(seurat,
ident.1 = "IPF",
ident.2 = "Control")

• are you showing the full function called? it should FindMarkers(seurat,group.by="group",ident.1="IPF",ident.2="Control") unless you set the identity to be group Jan 21 at 10:16