How do you verify if a RNAseq dataset is unstranded, stranded or revesely stranded from a fastq file?

  • $\begingroup$ I was looking for a method that didn't involve quasi-mapping but I think this will solve my problem. Thank you! $\endgroup$ Jan 27 at 9:15
  • 1
    $\begingroup$ Unless the metadata is recorded somewhere you won’t get around mapping: the FASTQs themselves don’t record this information anywhere. $\endgroup$ Jan 27 at 9:18

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