just a quick question about fast5 pass fail folders. I have been working under the assumption that the fast5 pass fail folders I was given as raw data from our ONT vendor came from them doing base calling with guppy 3.something. Is that right? (The person that worked for the vendor has since moved on so I can't ask them - so my question is really does guppy3 divide fast5s into pass/fail?)
I further assumed that given I am now doing my own basecalling with guppy4.4 that I should use all the fast5s (both pass and fail) as some of the fails might become pass with the improved NN. Again, am I right? If not could someone please explain?
I'm going to also put this question on the ONT community site, FYI