1
$\begingroup$

I trying to locally reproduce a Manta run as seen in the log of an illumina basespace RNA-Seq Alignment tool v1.1.1 run I've done. The log reads as follows:

[LOG][2021-03-12 20:58:56.377]03/12/2021,20:58:56.193,Launch process:  \
/path_to_RNA_Tools/bin/python  \
'/path_to_RNA_Tools/Manta/bin/configManta.py'  \
--rna --normalBam='path_to_alignments/alignments.bam' \
--runDir='path_to_samples/CTR/replicates/CTR/MantaFusions/manta.run'  \
--referenceFasta='/path_to_genomes/genome.fa' \
--config '/opt/illumina/Isis/2.6.25.18/Workflows/WholeGenomeRnaSeqWorker/scripts/configManta.py.ini'

The file shown in the line above configManta.py.ini is however no were to be found. The Manta user guide states in the linked section

Options listed in the file: ${MANTA_INSTALL_PATH}/bin/configManta.py.ini

The configManta.py is in the expected directory, only the configManta.py.ini is absent.

I am using Manta version 1.6.0 from bioconda.

Your insights will be much appreciated!

$\endgroup$
1
$\begingroup$

The --config file option for Manta can be used to override the defaults in the global config file. If you type configManta.py -h, you'll see where the global config file has been installed. For me, it's here:

/home/steve/miniconda3/envs/manta/share/manta-1.6.0-1/bin/configManta.py.ini

So the command in your log file takes a specific config file installed into:

/opt/illumina/Isis/2.6.25.18/...

I suspect 'Isis' is a reference to Illumina's analysis tool 'IsisRNA'. Papers that mention Illumina's 'RnaReadCounter' tool, say that it is part of the internal analysis tool 'IsisRNA'. Because it's one of Illumina's in-house tools, I think you'd need to contact Illumina support to get a copy. I would only do this though if you need to reproduce exactly as per the command in your log file. If you only need to roughly reproduce the output, the defaults in the global config file are probably fine.

$\endgroup$

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service, privacy policy and cookie policy

Not the answer you're looking for? Browse other questions tagged or ask your own question.