I want to use rna seq data to later perform functional tests on fusion genes. so before that I need to filter the "best results" (of rnaseq) for deciding which candidates I actually want to try in the lab. one criterium is the split read. (among coverage, discordant mate and confidence)
In some cases the split read (or discordant mate/coverage) of one of the two genes that fusioned is "0".
Does that automatically mean that the gene is no longer a candidate for functional testing?
Unfortunately I only got the results in an excel data sheet and don't know which tool was used to gain data.