where can I find what
7 mean in a Sequence file?
I was looking at this sequence file I got from NCBI (it's an sra file? if that is the correct terminology) and I used the
sratoolkit to print out a few
looking at them I expected to see ACGU (this was meant to be an RNA sequence), but what I see are like
Where can I find a reference to help me "understand" this?
I mean I assume
- means missed or unknown? or is it meant to be already aligned and these are deletions?
I appreciate any pointer to get started. as you must have observed I am a total noob.
I got the file from: https://www.ncbi.nlm.nih.gov/sra/SRX8156420[accn]
Then I did a
./fastq-dump -X 5 -Z ERR4557575
- Where do you look for the quality scores ranges and such, I guess as @winnie2k suggested I should convert this to FASTQ and make my life easier, but still wanted to understand the file better.