The student who was working on scRNA seq of KO and WT lines has made a mistake and he mixed both lines and generate the final sequencing data. Now, we are having gene expression data but don't know which is KO and which is WT.
It's a genetic KO. Looking at the presence of absence of the KO gene might not be helpful here as its not deep sequenced.
Cells were extracted from two stable line of animal; one wild type and other Chd2 gene knocked out. As a sequencing output I am having one fastq file in the format 28+10+10+90 . I can generate a count matrix out of it; but I dont know which barcode from WT and with one from KO.
Is there any way to segregate the output based on the KO gene expression?