I am reading this paper, where the authors mention

We apply SAUCIE to the batch correcting, denoising and clustering of an 11-million cell mass cytometry dataset with 180 samples from 40 subjects in a study of the dengue flavivirus

What exactly is the difference between a “sample” and a “subject” in scRNA-Seq?

PS: here’s a possibly relevant visual from the paper, but I still couldn’t understand the difference enter image description here


1 Answer 1


A subject is an individual or donor. You can take multiple samples from the same subject, e.g. from different tissues, different timepoints etc.


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