# TCR-seq or scRNA-seq

I need idea, intuition, suggestion please

I have 10X scRNA-seq of PBMC (multiome's 3' poly-A capture), can I capture TCR/BCR from my scRNA-seq or I need TCRseq ? I googled and likely TCR and BCR immune profiling require 5' transcript capture and the V(D)J variable sequence is on the 5' end of the gene. So the 3' capture typically yields the C constant region of TCR and BCR transcripts. can you help me please? Thank you

The 3' gene expression protocol will capture TCR and BCR mRNAs but this may not be very helpful to you. As you already mentioned only the 3' end will be sequenced, which are the constant regions. With this data you may potentially be able to discriminate TRAC from TRBC1 and so forth.