I have three bulk RNA-seq samples (test1, test2, test3) without replicates.
And I noticed that DEG analysis tools such as DESeq2/edgeR/etc cannot be applied for data with no replicates.
So, I just drew a heatmap with expression value (TPM) of genes of interest, using NMF (aheatmap).
But now I want to perform DEG analysis across the three samples. Is there anything I can try for the analysis?
My TPM data (.csv) looks like below.
Thank you so much.