# Gene expression changes in one condition

I want to look at an RNA-seq data-set on 1 condition to look for expressed genes. As there is only one condition, I can’t perform differential analysis.

The only thing I can think to do is set a threshold (i.e. all replicates must have > 10 gene counts) then say these are expressed. But I don’t think this is a very good way of doing it.

Do you know of any better ways that we can do this?

Thank you for any idea

vsd <- vst(dds, blind=FALSE)