Changing the order of colors in pheatmap

Question

I plotted a heatmap

I want cancer to be red and healthy to be blue

   pheatmap(heat1, annotation_col=df,color=colorRampPalette(c("navy", "white", "red"))(50))

You are seeing cancer is in blue in annotation, how I can change that

Answer 1

It is possible with annotation_colors argument :

First create a list with your conditions and then add the argument :

annot_colors=list(HealthStatus=c(Cancer="#F0978D",Healthy="#63D7DE"))
    
pheatmap(heat1,
         annotation_col=df,
         color=colorRampPalette(c("navy", "white", "red"))(50),
         annotation_colors=annot_colors)