So far I haven't done any variant calling as such. Nanopore I have used for 16s microbiome data.
Now My question/doubt so how do I proceed for nano-pore virus sequencing data
- I get fast5 files
- The i perform base-calling using guppy
- Get fastq files
Now after doing the above is it possible to use variant calling that is used for Ilumina once I get the fastq files?