I am analysing RNA-Seq data for a set of genes from different human brain regions using data from the Allen Brain Atlas. I have made a heatmap using GraphPad Prism. In this heatmap, I show the average RPKM value for each gene of interest for each brain region (the average is taken as there are multiple samples for each brain region).
I am new to creating heatmaps but I know that you can use different methods to cluster the heatmap so that the data is more easy to interpret. I want to cluster the heatmap so that the brain regions which have the highest level of gene expression overall are grouped together.
I have seen online that
R can also be used to create a heatmap, and I have had previous experience with
R. Does anyone have any recommendations on any good tutorials (that are not too difficult) on creating heat-maps in
R as well as clustering the data?