I have a large protein PDB file (~3500 residues) and a couple of PDB files containing glycan structures I sourced from GLYCAM.
How can I attach the N and O-glycans onto each protein residue with software? In PyMol, I am stuck at loading up the protein PDB file and glycan file together, but I don't know how to put the two elements together. Are there any commands I can use? or tutorial resources I can take a look at? I'm open to trying other platforms.
I've tried glycan modelling webtools (GLYCAM, CHARMM-GUI) but they can't seem to process my large glycoprotein - leading to errors or extended waiting times which also end in failure.