How does one go about comparing expression levels of one gene across multiple microarray datasets pulled from different experiments/papers in mice? In my example, I've downloaded the expression levels for my gene of interest from different microarray datasets for different cell populations of interest (specifically healthy hematopoietic cells). How would I pool the datasets together?
Say I have common cell types from multiple datasets such as B-cells, would I be able to just combine them into one data frame and visualize them as one or would I need to normalize some aspect of that?
I'm really confused because I've had different people tell me different things.