It is my first time using Chimera. I have uploaded a PDB file on to Chimera and opened it up on the software. I need to change the threshold of the EM map so that I can analyze what the EM map would look like with a threshold too high and too low.

Please let me know if you have any suggestions.


  • $\begingroup$ Could you supply more details on the goal of your analysis? $\endgroup$
    – M__
    Feb 11, 2022 at 14:22
  • 2
    $\begingroup$ cgl.ucsf.edu/chimera/current/docs/ContributedSoftware/… $\endgroup$
    – pippo1980
    Feb 11, 2022 at 14:22
  • $\begingroup$ I am trying to view a protein with a threshold that Is too high, a threshold that is too low, and finally, the correct threshold to se the secondary structures. $\endgroup$ Feb 11, 2022 at 14:32


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