I have about 300 90-bp sequences which I would like to map to a reference genome to make it longer to a 300bp sequence, wherein the 90bp is at the middle.
Anyone knows what bioinformatics tool I can use for this? Thank you!
Actual scenario: The SNP sequences are from a SNP panel data (90bp sequence, SNP is at the middle). We would like to extend the sequence to 300bp, retaining the SNP at the middle so we can make a sequence capture panel of the SNP.