I am in need to benchmark the variant identification pipeline which uses RNA seq data alone without any matched-normal. I would like to know the reference dataset (and the pipeline on which the dataset was run) that can I use to compare the pipeline results on my dataset. The reference dataset which I use should be comparable regardless of the dataset specific to certain disease. Thanks all in advance for your responses.

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    $\begingroup$ Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking. $\endgroup$
    – Community Bot
    Commented Oct 20, 2022 at 7:57
  • $\begingroup$ Well, what is your dataset? What species? What kind of sample? You mention the absence of a matched normal, does that mean your sample is from a tumor? We need to understand what you have in order to help you validate. $\endgroup$
    – terdon
    Commented Oct 20, 2022 at 10:07
  • $\begingroup$ The samples come from human related to diabetic condition $\endgroup$
    – Ahkam
    Commented Oct 21, 2022 at 8:57


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