I am utilizing
miniconda for RNA sequencing. I have successfully made an index; using said index to analyze RNA sequencing data has yielded an error of:
"Error: index input file could not be opened!"
The implication is that the code used does in fact work but raises the question as to why the index file could not be opened. I have tried searching this error to see if others may have faced the same error message, but unfortunately no luck. Any recommendations and or solutions would be greatly appreciated. The code and output can be found below.
kallisto quant -i Homo_sapiens.GRCh38.cdna.all -o test -t 8 --single -l 250 -s 30 SRR8668755_1M_subsample.fastq
[quant] fragment length distribution is truncated gaussian with mean = 250, sd = 30
Error: index input file could not be opened!