I have two NGS files from an NGS company corresponding to the sequencing data from a tumor sample as follows:

  • TB_7710391_R1.FASTQ.gz
  • TB_7710391_R2.FASTQ.gz

I have downloaded the genome for MCPyV as a file called “MCPYV_Sequence.fasta”

I have downloaded the Hg38 as a file called “hg_38.fa”

I have input the host genome as hg_38.fa, the viral genome as “ MCPYV_Sequence.fasta” and the sequence data as both FASTQ files.

I run the assembly in the cloud, and I receive back an error stating that the assembly as failed.

What am I doing wrong?

  • $\begingroup$ Please clarify your specific problem or provide additional details to highlight exactly what you need. As it's currently written, it's hard to tell exactly what you're asking. $\endgroup$
    – Community Bot
    Commented Feb 11, 2023 at 23:28
  • 1
    $\begingroup$ This question needs more detail. What cloud service are you using? How are you using it? Why are you trying to do an assembly? What results are you looking for? $\endgroup$
    – gringer
    Commented Feb 12, 2023 at 19:19


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