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When doing Illumina 2x150bp sequencing of genomic DNA, and after aligning the reads to GRCh38, what percentage of the non-N fraction of the human genome is MAPQ=0? This is, what part corresponds to regions that can't be uniquely mapped with 2x150bp reads.
And, how many genes are affected by MAPQ=0 regions?
I presume the numbers will dance around depending on fragment sizes, read quality, etc. but I am happy with some starting numbers.
Mapping quality is determined by the repetitiveness of the genome, the sequencing error rate, insert size, the capability of the mapper and the nasty heuristics behind the mapper. MAPQ=0 to one mapper is not necessarily MAPQ=0 to another.
That said, I get what you mean. You want to know the uniqueness/repetitiveness. It is still hard if you want to get a useful answer. For 150bp reads, each reference position is covered by 150 reads. What if 50 of them have no other exact hits elsewhere, but the rest 100 have? Is this position a repeat or not? In addition, what if the 50 are unique only because one mismatch? If there is a variant at that mismatch, the 50 would become repeats or mapped elsewhere.
My preference is to say a position is "unique" under k-long reads if over k/2 reads overlapping the position have no other perfect or 1-mismatch/1-gap hits elsewhere in the genome. Under this definition, 79.3% of human genome are unique for 35bp reads. 92.4% for 75bp reads. I don't have the number for 150bp reads. I guess it will be around 95%. Empirically, 94-95% of human genome is callable with 100bp paired-end reads.
As to other measurements, the most common one is the fraction of reads that has an exact hit elsewhere. You can use Fabio's method. It gives a good enough estimate once you simulate over 1 million reads. This fraction is around 86% for 35bp reads and 95% for 75bp reads, as I remember. The problem with this approach is this fraction is not very informative to variant calling due to the issues I talked about. Another way is to use RepeatMasker. It is worse. RepeatMasker masks 50% of human genome, but excludes segmental duplications where short reads can't be confidently mapped.
As has been said before, mappability to the 'human genome' depends on a number of factors, among these the reference version and type of reads, for which you are interested in GRCh38 and 2x150bp reads. Although I am not aware of numbers accounting for these particular reference and type of Illumina reads, the 1000 genomes project has provided the community with a similar and close estimation that you might be interested in considering regarding your inquiry.
Similar to your question, the 1K project estimated 'the proportion of the human genome that is less accessible to short reads'. In these estimates the human genome is GRCh37 and the types of reads in question are mostly 2x Illumina with a mixture of lengths with the longest being up to 250bp. In these estimates each base in the human genome is considered (and marked) 'less accesible' according to these criteria:
L - depth of coverage is much lower than average
H - depth of coverage is much higher than average
Z - too many reads with zero mapping quality overlap this position
Q - the average mapping quality at the position is too low
Each of these criteria has "standard" and "strict" thresholds for a base to be considered - or not - in each category. You can read more in the link below:
According to the strict thresholds, the human genome has about 16.8% of "Z" and 3.1% of "Q" bases, respectively. Considering the "Z" and "Q" criteria as a proxy for ~ mapq=0, about 19.9% of the human genome can not be uniquely mapped.
I would suggest you to run some simulation. You can use wgsim to simulate reads that are as much similar to what you want an answer for. I don't think there are faster methods.
